Junction League's Nikon X1#
Capabilities#
- Spinning disk confocal unit optimized for high resolution imaging
- Four lasers (405nm, 488nm, 561nm, 640nm)
- Live-cell imaging (5% CO2 and temperature control)
- Dual cameras for simultaneous two-channel confocal imaging
- Inverted Nikon Ti2-E with Perfect Focus System for eliminating focus drift
- Motorized stage and z-piezo stage allow for tiling/stitching and fast z-stacks
- Selective photoactivation/photobleaching with a 405nm laser
Check fluorophore performance on FPbase
Objectives#
| Mag. | NA | Name | Immersion | Condenser for DIC |
|---|---|---|---|---|
| 4x | 0.20 | Plan Apo Lambda | Air | N/A |
| 10x | 0.45 | Plan Apo Lambda | Air | N1 |
| 20x | 0.75 | Plan Apo Lambda | Air | N2 |
| 40x | 1.30 | Plan Fluor | Oil | N2 |
| 60x | 1.49 | Apo TIRF | Oil | N2 |
| 100x | 1.49 | Apo TIRF | Oil | N2 |
Sample holders#
| Type | Fixed imaging | Live imaging |
|---|---|---|
| Slides | ||
| 35mm dish | ||
| 60mm dish | ||
| Well plate |
Scheduling and Training#
Scheduling: See Teamup calendar
Training: Email William Giang