Troubleshooting

Microscope does not turn on properly

This can be heard (by the microscope beeping) and also seen (by the joystick pad not turning on). Solution: Turn off the microscope body and turn on again. It should work within three tries.

Parts of the microscope don't respond to Elements

Solution: Try restarting the software and microscope body.

Elements crashed and FRAP doesn't work any more

Solution: The microscope body must be power cycled in addition to restarting Elements.

Switching between OCs takes a while

If OCs have different camera settings (e.g. readout mode or bit-depth BUT NOT EXPOSURE TIME), it takes a long time for the camera to change settings.

Can't see blue or green signal through computer in single-camera mode

Check to see if the TwinCam dichroic mirror is in. If it is, remove it.

Removing the TwinCam cover reveals that the dichroic mirror is in

Common Pitfalls

Do not place coverslips too close to the edge.

Coverslips too close to the edge will be troublesome, especially if using immersion objectives.

Do not get close to the stage insert edge during live imaging with immersion objectives if you want multipoints