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Changes (software and hardware)#

Oct 5 2023#

  • The Bioptechs Objective Heater (Medium) and controller were tested by Will and Nav and confirmed to stably heat the sample at 37C.

Oct 3 2023#

  • An unused filter holder within the CSU-X1's emission filter wheel became stuck. Unused filter holders were removed (along with the original cracked polychroic).
  • NIS-Elements updated from v5.30.06 to v5.42.03 for better stability.
  • Lauren and Fred recentered the GalvoXY miniscanner using the 100x/1.49 objective.

June 2 2023#

  • An ND filter which permits 10% transmission has been inserted into one of two slider positions, impacting only the SOLA unit. This was a cheap and fast alternative to sending the SOLA unit back to the factory for a firmware update that permits setting the SOLA power before 7% without blinking. One example of when you'd want a lower SOLA power is when scanning for suitable cells for live-cell imaging.
Slider location for whether the ND filter is in or out of the light path

ND filter in

ND filter out

May 9 2023#

  • Lauren and Tim "rebuilt" the scope in Elements without any change to the version of Elements.
  • Except for unplugging the UNIT2 cable responsible for moving the 50/50 beamsplitter in the L-APP unit, no hardware was touched.
  • Oddly enough, a complete rebuild of the system within Elements re-enabled "Triggered Acquisition", but trying to re-load previous device settings ended up breaking "Triggered Acquisition". We settled on a separate Platform folder for people who need to use "Triggered Acquisition" which will be swapped in/out when finished with the default Platform folder.
  • An ND filter has been ordered in order to deal with the blinking SOLA unit when set below 7%.

February 10 2023#

  • Our repaired 100x/1.49 Apo TIRF objective is back on the scope, and Tim took the loaner.

July 18 2022#

  • Nikon moved the scope to its new home in C7768G
  • NIS-Elements updated to v5.30.06 from v5.30.04
    • Bug fix for multi-point z-stacks: The first timepoint of the first multipoint no longer has duplicated slices.
  • Using a loaner 100x/1.49 Apo TIRF objective (Thanks Nikon!)

January 12 2022#

  • Nikon installed the L-APP 50/50 BS with a primary goal of reducing the power of the 405nm FRAP laser.